Isolation of Plasmid Dna by Alkali-Lysis Method

1. INTRODUCTION 1.1 PLASMID plasmid DNAs are extra chromosomal hereditary material. They are rotund molecule of DNA that led an free existence in b make uperial cell. plasmid DNA has exhaust hood skill of self replication or may be integrating with bacterial chromosome. Plasmid has at least single DNA sequence that can act as an germ of replication. Plasmid almost always give birth unmatchable or more gene which is responsible for a reclaimable characteristic displayed by bacteria .e.g. Antibiotic protection gene, then it is used as selectable marker. All plasmid DNA behave atleast one DNA sequence that can act as an origin of replication, so they are able to visualise with the cell quite independently of the main bacterial chromosome. The short plasmids make use of the host cells desolate DNA replicative enzymes in score to make copies of themselves, whereas some of the walloping ones carry genes that code for special enzymes that are specific for pl asmid replication. Size and copy number of plasmid is an important be in possession of of plasmids for cloning purpose e.g. plasmid ranging from 6-10 Kb is very satisfying for cloning and having copy number as many as 50. Plasmid may be of following type: F-Plasmid: having exponent to promote conjugal transfer of plasmid e.g. F-Plasmid of E.

coli R-Plasmid: responsible for providing foeman to host against foreign bodies such as anti bacterial resistance e.g. RP4 of Pseudomonas Col-Plasmid: codes for colicins, protein that kills other bacteria e.g. ColE1 of E.coli and Pvocine of Pseudomonas Degradative Plasmid: it allows the bacteria to metabolize unusual molecule ! such as toluene and salicylic venereal disease e.g. TOL of Pseudomonas putida Virulence Plasmid: it confers pathogenicity on the host bacterium e.g. Plasmid of Agrobacterium. Essential features of an exemplar plasmid cloning vector sharp size: dispirited molecular weight, are easier to handle, present in multiple copies, do not get damaged during isolation, surmount through cell...If you indispensability to get a full essay, order it on our website: BestEssayCheap.com

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